Celia Heyes

نویسنده

  • Celia Heyes
چکیده

can be controlled precisely. In the experiment reported, they designed this distance to be 23 nm, which they describe as “a compromise of strong fluorescence enhancement and sufficient space for accommodation of a biomolecular assay.” In the ‘hotspot’ between the two particles, Acuna and colleagues introduced a docking site for molecules of interest. At this site, they bound the dye ATTO647N for a range of fluorescence experiments including single-molecule fluorescence resonance energy transfer (FRET) studies. They could confirm that the nanoparticles enhance the fluorescence signals more than 100-fold, making single-molecule studies possible at micromolar concentrations, corresponding to typical biological samples. They could also follow the time course of binding and unbinding of the dye-carrying DNA strand.

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عنوان ژورنال:
  • Current Biology

دوره 23  شماره 

صفحات  -

تاریخ انتشار 2013